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Antioxidant nanozymes are therapeutic candidates for oxidative diseases. Here a standardized protocol is described to characterize superoxide dismutase-like or catalase-like nanozymes and quantify their activity.
A microscopy approach for the use of focus-feedback for single-plane tracking of subcellular structures during prolonged live-cell fluorescent imaging sessions.
This Protocol Extension describes a programmable tetrahedral DNA nanostructure (TDN) platform for selective cell and protein capture, using aptamer-functionalized TDN for cell capture and peptide-functionalized TDN–hydrogel for protein sequestration.
Our authors are invited to write blog posts that describe how they conceived and developed their protocols, prior to publication at Nature Protocols. These stories are published on a community website for researchers who are interested in techniques and methods.
[FeFe]-Hydrogenases catalyze H2 production and consumption. Their impact is limited by the complexity of the natural synthetic process. This protocol describes the synthesis of diiron subclusters to simplify the production of functional enzymes.
An approach for combining expansion microscopy with fluctuation-based super-resolution analysis enables the visualization of individual proteins via conventional fluorescence microscopy.
A printable composite material provides a practical and cost-effective route for fabricating metasurfaces on flexible or curved substrates, scalable from the UV to IR wavelengths and deployable for photonic devices.
Antioxidant nanozymes are therapeutic candidates for oxidative diseases. Here a standardized protocol is described to characterize superoxide dismutase-like or catalase-like nanozymes and quantify their activity.
Stabilization of metal single-atom or cluster catalysts is achieved by forming CeOx nanoislands on SiO2 through strong electrostatic adsorption method. Transition-metal precursors are added to form single atoms or clusters on these islands.
A microscopy approach for the use of focus-feedback for single-plane tracking of subcellular structures during prolonged live-cell fluorescent imaging sessions.